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Family Sterculiaceae / Malvaceae
Theobroma cacao L.

Ke ke shu

Scientific names Common names
Cacao minar Gaertn. Kakaw (Tag.)
Cacao minus Gaertn. Cacao tree (Engl.)
Cacao sativa Aubl. Chocolate tree (Engl.)
Cacao theobroma Tussac Cocoa tree (Engl.)
Theobroma cacao L. Food of the Gods (Engl.)
Theobroma integerrima Stokes  
Theobroma kalagua De Wild.  
Theobroma leiocarpum Bernoulli  
Theobroma pentagonum Bernoulli  
Theobroma saltzmanniana Bernoulli  
Theobroma sapidum Pittier  
Theobroma sativa (Aubl.) Lign. & Le Bey  
Theobroma sativum (Aubl.) Lign. & Le Bey  
Theobroma cacao L. is an accepted name The Plant List

Other vernacular names
CHINESE: Ke ke shu
DANISH: Kakaotrae.
DUTCH: Cacaoboom.
FINNISH: Kaakao, Kaakaopuu.
FRENCH: Cacao, cacaoyer.
GERMAN: Kakaobaum, Kakaopflanze.
HINDI: Kokko.
ITALIAN: Albero del cacao.
JAPANESE: Kakao, Kakao no ki, Kokoa no ki, Teoburaama kakao.
KHMER: Kakaaw.
MALAY: Pokok coklat.
MALAYALAM: Kokko, Kokkoo.
NORWEGIAN: Kakaotre.
POLISH: Kakaowiec.
PORTUGUESE: Arbore de cacao, Arvore-da-vida, Cacau, Cacau-de-mata, Cupuacu de mata.
RUSSIAN: Kakao, Shokoladnoe derevo.
SINHALESE: Maikonagaha.
SPANISH: Arbol de cacao, Cacahualt, Cacao amarillo, Cacao del monte, Cacaoeiro, Cacaotero.
SWEDISH: Kakao, Kakaobuske.
TAMIL: Kakkavo, Kona maram.
THAI: Kho kho, Ko ko.
TURKSIH: Hint bademi agaci, Kakao agaci.

Etymology / Gen info
Chocolate comes from the fruit of the kakaw tree. Kakaw's scientific name "Theobroma" means "food for the gods," derived from the Greek words theo (god) and broma (drink). In the Aztec language, the drink was called chocolati. In pre-Columbian times, its bean was a major currency with great trading value.
• Chromatographic analyses of residues from pottery vessels show that cacao beverages were being made before 1000  B.C., extending confirmed use of cacao in Mesoamerica back at least 500 years. (54)
• The Aztecs regarded Xocoati as a powerful aphrodisiac and stimulating tonic. Moctezuma, the 9th ruler of Tenochtitlan, is said to have indulged in a dose before entering the royal harem. (41)
• The current global production of cocoa beans is estimated at 3,520,000 tonnes while grinding is estimated at 3,678,000 tonnes for year 2008/09. Africa yields about 70% of cocoa production. Ivory Coast is the leader in cocoa production followed by Ghana and Indonesia. (12)
• Seeds are the most valuable part of the plant, providing source material for the production of chocolate. Trees start to produce fruit after five years and can live for over 200 years. However, they are considered commercially productive for only about 25 years. (41)
•  There are three main cultivar groups of cacao beans used to make cocoa and chocolate: (1) Criollo: The cocoa tree used by the Mayas, highly prized and rare, less bitter, more aromatic, source of only 5-10% of chocolate made. (2) Forastero: The tree includes several sub-varieties, significantly hardier than Criollo tress, producing cheaper cocoa beans, used for making 80% of world chocolate production. (3) Arriba: Considered the best variety, one of the few cultivars suited to produce fine-flavor chocolate. Trinitario is a hybrid of Criollo and Forastero, used fro about 10-15% of chocolate production. (54)
•  Chocolate is considered the third highest contributor of antioxidants to the American diet, 100-107 mg/day; compared to fruits at 255 mg/day, and vegetables 233 mg/day. (54)

Kakaw is a small evergreen tree with a globose crown, growing to 5 to 8 meters high Leaves are alternate, entire, oblong-ovate to oblong, 15 to 40 centimeters long, 5 to 20 centimeters wide, with pointed tip and rounded base. Flowers are solitary or fascicled on the trunk and branches; yellowish or nearly white, about 1 centimeter in diameter. Fruit is oblong, 10 to 15 centimeters long, prominently wrinkled, yellow or purplish. Seeds are numerous and embedded in whitish pulp; when ripe they rattle in the capsule when shaken.  

- Widely scattered in cultivation at low and medium altitudes.
- Cultivated for its seeds.
- Nowhere spontaneous in the Philippines.
- Introduced from Mexico.

- Cocoa contains approximately 380 known chemicals and 10 psychoactive constituents.
- Seeds contain fixed oil, 40-56 %; theobromine; glucose, saccharose; vitamin A, 825-1400 I.U. per 100 gm; cellulose, 2.8-5.4%; water, 5-7%; ash, 3-5%; starch, 5% and a glucoside, cacarine.
- Seeds yield about 2% theobromine, 40 to 60% solid fat. Shell contains about 1 percent theobromine.

- Each seed yields a significant amount of fat (40-50% as cocoa butter) and polyphenols which make up 10% of the whole bean's dry weight. (13)
- The mesocarp and seed contain theobromine and caffeine.
- The wall and pulp of the fruit contain arabinose and galactose.
-The flesh contain enzymes: protease, invertase, raffinase, cesease and oxidase.
- Cacao is high in magnesium.
- High in antioxidants, approximately 40 times higher than blueberries.
- Possibly contains MAO inhibitors with effects on serotonin and neurotransmitters.
- Contains PEA (phenylethylamine) and anandamine.

- High in polyphenols, with three main groups: catechins (37%), anthocyanins (4%) and proanthocyanidins (58%). The main catechin is (-)-epicathechin with up to 35% of polyphenol content. (11)
- Studies have yielded various polyphenolic compounds, viz., simple phenols, benzoquinones, phenolic acids, acetophenones, phenylacetic acids, hydroxycinnamic acids, phenylpropenes, coumarines, chromones, naphthoquinones, xanthones, stilbenes, anthraquinones, flavonoids, lignans, and lignins.   (11)
- Study of phytochemical constituents of coca fruit outer shell yielded fats and oils +, steroids ++ , alkaloids +. tannins +++, phenolic compounds +++, cardiac glycosides +, saponins ++, flavonoids +++, proteins +++, tyrosine +, carbohydrates +, reducing sugars +, tannic acids ++, gums +++. (29)
- Crude ethanol extract of stem bark yielded alkaloids, tannin, saponin, glycoside, phenol, flavonoid, and carboxylic acid. (see study below) (32)
- Nutrient values of dark chocolate, 70-85% cacao solids, per 100 g yield: (Proximates) water 1.37 g, energy 598 kcal, protein 7.79 g, total lipid (fat) 42.63 g, carbohydrate by difference 45.90 g, total dietary fiber 10.9 g, total sugars 23.99 g; (Minerals) calcium 73 mg, iron 11.90 mg, magnesium 228 mg, phosphorus 308 mg, potassium 715 mg, sodium 20 mg, zinc 3.31 mg; (Vitamins) thiamin 0.034 mg, riboflavin 0.078 mg, niacin 1.054 mg, vitamin B6 0.038 mg, vitamin B12 0.28 mg, vitamin A 2 µg, vitamin A 39 IU, vitamin E 0.59 mg, vitamin K 7.3 µg; (Lipids) total saturated fatty acids 24.489 g, total monosaturated FA 12.781 g, total polyunsaturated FA 1.257 g, total trans FA 0.030 g, cholesterol 3 mg; (Others) caffeine 80 mg. (48)
- Each seed contains a significant amount of fat (40-50% as cocoa butter) and polyphenols, which make up about 10% of the whole bean's dry weight. Epicatechin concentrations among freshly harvested beans range from 21.89 to 43.27 mg/g of dry defatted samples.   (54)
- Study evaluating the volatile composition of cocoa from 16 accessions from three different morphogenetic groups, namely, Criollo, Forastero, and Trinitario profiled 53 compounds. Only a few compounds were found exclusively specific to one group, Trinitario. Trinitarion contained high contents of furfuryl alcohol, 3-carene, 2-pentanol, 1-pentanol, 2,3-butanediol, 2-heptanol, and benzyl acetate. Criollo cocoa contained high amounts of α-limonene, ß-caryophyllene, ß-myrcene, α-phellandrene, ß-linalool, and acetic acid. Forastero yielded high amounts of 3-methylbutanoic acid, 2-(2-butoxyethoxy) ethanol, anethole,  and 2,4-pentanediol.  (55)

- Considered emmenagogue and ecbolic.
- Emollient, diuretic, aphrodisiac, nutritive.
- Studies have suggested anti-carcinogenic, anti-atherogenic, anti-ulcer, anti-thrombotic, anti-inflammatory, immune modulating, anti-microbial, vasodilatory and analgesic properties.
- Theobromine resembles caffeine in action, with less powerful effects on the central nervous system.
- Rich source of polyphenols, reportedly with higher antioxidant activity than teas and red wines.

- Oil of Theobroma or cacao butter is a yellowish white solid, with odor resembling that of cocoa, tasting bland and agreeable. (18)
- The feel good sensation with chocolate is attributed to the chemical phenylethylamine which might be partly responsible for the release and potentiation of brain dopamine. Higher concentrations of PEA are found in some cocoa beans and high quality cocoa powder.

Parts utilized
Seed, roots, oil, bark, flower, fruit pulp, leaves.

Edibility / Culinary
- Cultivated for use in the manufacture of cacao, chocolate, cacao butter, chocolate food, drink or fruit.
- Ro
asted seeds used as snack food.
- In Zamboanga, del Sur, Philippines, poultice of young fruits applied to boils and skin inflammation. (59)
- Oil or cocoa butter is an excellent emollient, used to soften and protect chapped hands and lips.
- Eczema, dry skin: Roast 10-12 seeds and pound ; apply to affected areas as poultice after a warm compress.
- Root decoction used as emmenagogue (promotes or stimulates menstrual flow) and ecbolic (promotes labor by stimulating uterine contractions.
- Husk is traditionally used to treat the pains of pregnancy, fevers, and coughs.
- Pod of T. cacao and shaft of Elaeis guinensis are burned together, poured into a water container, and used to bathe kids infected with craw-craw (itchy skin disease caused by larvae of filarial worm causing onchocerciasis migrating to the subcutaneous tissues). (38)
- In Nigeria, leaves used for wound healing, as worm expeller, and treatment of malaria.
- In southwest Nigeria, decoction of bark of Theobroma cacao and leaves of Sorghum bicolor in 250 ml of
water taken twice daily for treatment of anemia. Bark of T. cacao boiled in water and mixed with hot pap as baby food. (60)
- In Ghana, leaf decoction used in traditional medicine for treatment of malaria.  Leaf also used to treat asthma, weakness, diarrhea, fractures, loss of appetite, pneumonia, cough, colic, poisoning, and parasites. (61)
- In Trinidad and Tobago, used for kidney problems.
- In
Douala, Cameroon, used for wound healing, depression, and stomach aches. (68)
- Cocoa butter: Cacao butter (oil of theobroma) is an excellent emollient for use to prevent chapped lips and hands.
it is used in the manufacture of confections, toilet articles and cosmetics; in pharmacy, used for pill coating and suppository preparation.
- Fuel: The wood--light, soft, and of low durability--is of little value. Occasionally used for making charcoal. The cocoa bean testa is used for fuel. (42)
- Ceremonial Food: Cacao was a tree and food most prized by ancient Maya and Aztec, consumed during rituals and offered as sacraments to the gods.
- Preparation:
Cocoa is prepared by grinding the beans into a paste between hot rollers, then mixing it with sugar and starch, with part of the fat removed. Chocolate is prepared in the same way, with the fat retained.

Hypoglycemic / Polyphenol Rich:
Study showed that Malaysian cocoa polyphenol extract has a potential of being an insulin-mimetic agent. Further studies are suggested to elucidate on the underlying mechanisms for its glucose reduction and insulin mimicking activities. (2) In an acute and chronic animal study, the administration of cocoa extract reduced overall blood glucose in normal, obese, obese diabetic and diabetic rats. The polyphenol rich content is believed to have contributed to the outcome. (34) Study evaluated the hypoglycemic properties of Malaysian cocoa polyphenols extract in-vivo and in-vitro. In-vivo study in diabetic rats showed significant reduction of plasma glucose. In-vitro study showed significantly increased insulin secretion compared to control. Results suggest a potential for the polyphenols to be utilized to lower plasma glucose and stimulate insulin secretion in type 2 diabetic patients. (36)
Anti-Ulcer: Effects of polyphenol substances derived from Theobroma cacao on gastric mucosal lesion induced by ethanol: Study
suggests that the antiulcer mechanism of the polyphenols was from radical scavenging and modulation of leukocyte function. (3)
Immune Activity:
Study evaluated the effect of (-)-epicatechin and cocoa extract on the activation of a lymphoid cell line. Extract down-modulated T lymphocyte activation and the acquired immune response which could be important in immune system reactivity such as autoimmune or chronic inflammatory disease. (5)
Flavonoids / Nitric Oxide / Endothelial Function: (1) Study indicate flavanol-rich foods provide extraordinary health benefits. In populations that no longer consume large quantities of such foods, the risk of cardiac and cancer deaths have significantly increased.
Flavonoids / Decreased BP and LDL: Studies have suggested the antioxidants and flavonoids in dark chocolate with benefits of lowering effects on blood pressure and LDL cholesterol levels.
Epicatechin: Epicatechin, one of the bioactive nutrients in cocoa can promote blood vessel relaxation and the cardio-benefits might not be antioxidant dependent.
Human Platelet Reactivity Modulation / Platelet Function Inhibition: Study sought to evaluate whether a 28-day supplementation with cocoa flavanols and related procyanidin oligomers would modulate human platelet reactivity and primary hemostasis and reduce oxidative markers in vivo. Results showed significant increase in plasma epicatechin and catechin concentrations and significantly decreased platelet function.
Antioxidative Polyphenols: Study isolated clovamide, deoxyclovamide, quercetin and its glucoside. In the bulk oil system, clovamide had the strongest antioxidative activity. Results suggest that chocolate is stable against oxidative deterioration due to the presence of these polyphenolic compounds.
Inhibition of NO Release / Cytokine Secretion Inhibition: Study shows that cocoa flavonoids not only inhibit NO release from macrophages but also down-regulate inflammatory cytokines and chemokines.
Polyphenols / Antioxidative Activity: Study showed the polyphenol content and its antioxidant capacity vary among a wide range of cocoa and chocolate products, with processing making a great impact on the level of
polyphenols. (11) Dark chocolate with its high cocoa content (>35%) is considered to have the richest polyphenols content in the group of cocoa derivatives. (13)
Hepatoprotective / Apoptosis Prevention / Autophagy Induction: Study shows that cocoa inhibits drug-triggered liver cytotoxicity and prevents apoptosis by inducing autophagy. Results suggest that cocoa can be added to the list of natural chemopreventive agents with a potential for hepatopathy prevention and therapy. (9)
Antioxidant and Biologic Activities / Cocoa
Hulls: A supercritical CO2 extraction method shows cocoa hulls by-product to be a matrix rich in fiber (pectin) and phenolics. A better characterization of the bioactivity of the phenolic pigments is suggested for its potential use in food technology as functional colorant ingredient or antioxidant complex extract. (12)

Colon Cancer Benefits: Study evaluated cocoa's effect in colon cancer, both in-vivo and in-vitro. Several preclinical studies concluded that dietary polyphenols, in large amounts, can exert a desirable effect. Cocoa is a food rich in polyphenols (flavonoids and phenolic acids). Its main flavonoids are flavan-3-ols, epicatechin, and catechin. Total polyphenols in raw cocoa is up to 60% in monomeric and oligomeric forms. In-vivo studies, demonstrated an antiproliferative effect of cocoa-rich diet. In-vitro studies were done on caco-2 cell line, considered as human epithelial colonic adenocarcinoma cells. Crude procyanidin and procyanidin-enriched extracts showed an inhibitory effect on G2/M phase of cell cycle, leading to non-apoptotic cell death. Studies have shown potential inhibition on pro-inflammatory mediators on TNF-α-sensitized Caco-2 cells. Study concludes suggesting large scale, long term, randomized, placebo-controlled studies. (14)
Activated Carbon from Pod Husk / Arsenic Adsorption: Study showed cocoa pod husk material, a waste biomass, can be used to produce activated carbon by chemical activation and ZnCl2 showed to be the best chemical activation agent. The activated carbon can adsorb arsenic (As), up to removal levels of 80% in less than an hour. (15)
Cacao and Cardiovascular Health: Review summarizes the available data on the cardiovascular effects of cocoa, highlighting its potential clinical implications associated with consumption. Possible mechanisms of its protective effects include (1) endothelial Function and NO (2) antioxidant properties (3) platelet function (4) anti-hypertensive effect (5) antiatherogenic effects including effects on insulin resistance and blood lipids. (17)
Phenylethylamine (PEA) / Pros and Cons: (1) Phenylethylamine is a natural alkaloid, related to amphetamines, functioning as a central nervous system neurotransmitter or neuromodulator. (2) The feel good sensation with chocolate is attributed to the chemical phenylethylamine which might be partly responsible for the release and potentiation of brain dopamine. Higher concentrations of PEA are found in some cocoa beans and high quality cocoa powder. (3) Depending on the type of chocolate, a 100 /day of chocolate consumption provides between 0.36-0.83 mg/day of Beta-PEA. (3) PEA is also believed to increase the release of AcH (acetylcholine), possibly with mood and cognitive benefits. (4) Although sold as dietary supplement, some believe oral PEA is ineffective because of extensive presystemic metabolism. (5) Synthetic Beta-PEA at doses of 0.63 to 1.35 mg/day has been reported to cause Parkinson's symptoms through by-passing of presystemic metabolism. (6) The concerns relate to synthetic PEA additives—hybrid or GMO—not naturally occurring PEA, escaping enzymatic metabolic action, reaching the brain in trace amounts. (6) When initial PEA level is low, enzyme inhibitors can raise it 1000-fold; 3 to 4-fold when initial concentration is high. Long-term effects of unmetabolized beta-PEA from daily ingestion are unknown. (18) (19)
Cacao Flavonoids on Immune Activation of Lymphoid Cell Line: Study evaluated the effect of (—)-epicatechin and cocoa extract on activation of lymphoid cell line. There was dose-dependent reduction of IL-2Ra (CD25) expression on activated cells. There was also IL-2 secretion inhibition and 3 to 4.5-fold increase in IL-4 release. In summary, the extract down-modulated T lymphocyte activation and the acquired immune response, which suggests a potential use in immune system hyperactivity such as autoimmune of chronic inflammatory disease. (20)
Antiproliferative / Leaf: Study evaluated the potential anticancer properties from non-edible parts of the cocoa plant, viz., leaf, bark, husk, fermented and unfermented shell, root, cherelle, and pith. The hexane partitioned fraction of cocoa leaf showed the highest anticancer activity with IC50 value about 66.7± 0.71 µg/ml and generated 10 major active compounds with synergistic effect against MCF-7. (21)
Effect of Roasting on Contents of Cocoa Beans: Study evaluated the effect of roasting conditions on the content of fat, tocopherol, and phytosterol and antioxidant capacity of the lipid fraction from cocoa beans. Results showed roasting significantly affected phytochemical composition and lipophilic antioxidant activity. Roasting may cause significant degradation of α-tocopherol and phytosterols compared to raw cocoa beans. (22)
Clovamide / Antioxidant Activity: Study identified the caffeoylated amino acid clovamide [( - )-N-[3'-4' -dihydroxy-(E)-cinnamoyl]-dihydroxyphenylalanine] in the antioxidant polyphenolic fraction of cocoa and investigated the effect of roasting on its content in different samples of cocoa beans. Although roasting was found to be detrimental for the clovamide content, no correlation was found between clovamide concentration and overall antioxidant properties of the cocoa samples, suggesting clovamide is important but not critical for the antioxidant activity. (23)
Bioactive Compounds for Skin Health: Cocoa is rich in bioactive compounds: polyphenols, theobromine and
minerals. Lines of evidence support the role of cocoa in the promotion of human health, and a full understanding of the mechanisms of cocoa-derived phytochemicals as modulators of cell signaling is key to the evaluation of the potent biomolecules as anti-aging agents. (25)
Effect of Cocoa Powder on Biologic and Hematological Parameters: Study evaluated the effect of consumption of cocoa powder on biochemical and hematological parameters in rat. Results showed significant reductions in total serum cholesterol levels, LDL-C and triglycerides with a significant increase in white blood cells. (26)
Antioxidant / Cocoa Oil and Cake: Study evaluated the antioxidant activity of cocoa oil and cake using DPPH, hydroxyl radical generated from H2O2, and peroxide oxidation by ferric thiocyanate method. Results showed significant antioxidant activity (P>0.05) compared to antioxidant standards of BHA, ascorbic acid and α-tocopherol. (27)
Review on Cocoa and Chocolates: Study reports on the composition, bioavailability, and comparative analysis of other food products, and their implications for cardiovascular disease and the immune system. Chocolate contains a high amount of saturated fats; however, the two major fatty acids, palmitic and stearic acids, appear to have fewer implications for progression of coronary artery disease than other saturated fats. (28) Chocolates is considered the third highest contributor of antioxidants to the American diet: 100-107 mg/day compared to fruits (255 mg/day) and vegetables (233 mg/day). (13)
Cacao Herb Drug Interactions: (1) Caffeine: Although cocoa contains small amounts of caffeine compared to other caffeine-containing herbs, when taken in sufficient quantities, cocoa can produce levels of caffeine sufficient to cause interactions. (2) Anticoagulant or antiplatelet drugs: Cocoa flavanols might have antiplatelet effects, and may be additive with aspirin. (3) Antihypertensives: Dark chocolate may decrease blood pressure, although in large quantities, the caffeine in cocoa may have the opposite effect. (4) Iron: Cocoa may reduce the absorption of iron. (30)
Antimicrobial / Pod Husk: Spontaneous aerobic fermentation of cacao husks yields a crude husk extract with antimicrobial activity. The CHE was found effect against yeast Saccharomyces cerevisiae and Moniliophthora perniciosa. Subfractions showed strongest antibacterial activity against S. cholerasuis and S. epidermis. (31)
Antibacterial / Stem Bark: Crude ethanol extract of stem bark yielded alkaloids, tannin, saponin, glycoside, phenol, flavonoid, and carboxylic acid. The crude extract showed antibacterial activity against four human pathogens viz., E. coli, P. aeruginosa, S. pneumoniae and S. aureus. (32)
Cardiac Benefits / Decreased DNA Methylation of Leukocytes: Study concludes cocoa consumption decreases global DNA methylation of peripheral leucocytes in humans with CVD risk factors. (33)
Immunomodulatory: In a study of aqueous and ethanolic extracts of cacao on IFN-γ,
neopterin and Kyn/Trp concentrations in mitogen-stimulated human peripheral blood mononuclear cells, breakdown of tryptophan by IDO, and formation of neopterin and IFN-γ were dose-dependently suppressed. The inhibition of tryptophan breakdown by cacao constituents could be relevant not only for immune system restoration, but also in its contribution to mood elevation and improvement in quality of life. (36)
Amelioration of Oxidative Stress, Hyperglycemia, and Dyslipidemia in T1DR: Study evaluated an ethanolic extract of T. cacao bean extract for ameliorative effects in hyperglycemia and dyslipidemia in type 1 diabetic rats. Short term administration of bean extract caused substantial reduction in blood glucose but did not obliterate hyperglycemia. In the study form and doses, the extract exhibited comparative limited capacities to reduce oxidative stress and ameliorate dyslipidemia in T1-DR. (37)

• Phenolic Compounds and Cancer Cell Lines Tested: Review lists the phenolic compounds found in Theobroma cacao and the biological effects and cancer cell lines tested: (1) Polymer procyanidins / Caco-2 (colon) (2) Procyanidin B2 / Caco-2 (colon), HL-60 (leukemia) (3) Epicatechin / Caco-2 (colon), SH-SY5Y (neuroblastoma), HepG3 (hepatoma), MCF-7 (breast) (4) 3'-O-methyl epicatechin / FEK4 (skin fibroblasts) and (5) Catechin / HepG2 (hepatoma), Caco-2 (colon), Int-407 (intestine). (40)
• Anti-Hyperglycemic Effect of Polyphenols / Seed: Study investigated the anti-hyperglycemic potentials of polyphenols extracted from fermented and unfermented Theobroma cacao seeds in Wistar rats with STZ-induced diabetes. Results showed anti-hyperglycemic effect--the 150 mg/kbw of fermented polyphenol was more potent and efficient in reducing activities of carbohydrate-degrading enzymes. (43)
• Antimalarial Potential / Antiplasmodial / Leaves: Study evaluated three plants viz. Persea americana, Theobroma cacao, and Tridax procumbens for phytochemistry and antiplasmodial activity. Aqueous extract of leaves showed antiplasmodial activity. The aqueous extract of Theobroma cacao was most active and was more active against W2 than 3D7 Plasmodium falcifarum. (44)
• Antimicrobial / Antioxidant / Cytotoxicity / Seed, Leaf and Pod: Study evaluated cocoa leaf, seeds, and pod extracts for antimicrobial and anticancer activity. Seed extracts showed zone of inhibition against pathogens Serratia marcescens, Staphylococcus aureus, Salmonella sp. and Shigella dysenteriae. The extracts showed good amount of antioxidant activity. On cytotoxicity assay against MG63 osteosarcoma cell lines, all extracts showed deterioration in the cell lines, but the pods extract showed maximum inhibition. (45)
• Antitumor Activity / Antioxidant / Murine Lymphoma Model / Seeds: Study evaluated protein fractions of cacao seeds for antitumor activity on a lymphoma murine L5178Y model. Antioxidant activity was evaluated by ABTS and ORAC-FL assays. The albumin fraction showed antitumoral activity as evidenced by a significant decrease (p<0.05) in ascitic fluid volume and packed cell volume. Highest antioxidant activity by radical scavenging was shown by the albumin and glutelin fraction in both methods assayed. (46)
• Comparative Phytochemical Analysis of Cocoa and Green Tea: Study evaluated the phenols and flavonoid content and antioxidant capacity in cocoa and green tea. Results reveal that one serving of cocoa has higher amount of polyphenols than that of green tea. Cocoa yields 550 mg of total phenol (GAE) and 566 mg of flavonoids (ECE) compared to green tea with 168.8 mg of total phenol (GAE) and 353 mg of flavonoids (ECE). Percentage of oxidant scavenging capacity was significantly greater in cocoa than green tea. Study suggests cocoa has more beneficial effects for combating diseases like cancer and cardiovascular diseases and for boosting the immune system. (47)
• Antiproliferative / Antioxidant / Roots and Leaves: Study evaluated the antioxidant and antiproliferative activity of Theobroma cacao methanolic extracts of leaf, bark, husk, fermented and unfermented shell, pith, root, and cherelle. Using DPPH, TBARS, and Folin-Ciocalteu assays, the root extract showed the highest antioxidant activity, with total phenolic content of 22.0 GAE/100 g extract compared to extracts of other plant parts. The MTT assay showed the leaf extract to have highest antiproliferative activity against MCF-7 cells with MIC of 41.4 µg/mL. It showed cytotoxic effect in cancer cells, but not in normal cells. (49)
• Antidiabetic / Antioxidant /  Antibacterial / Pod Husk: Study evaluated the antibacterial, antioxidant, and antidiabetic activity of three Indonesian plants.: Theobroma cacao, Anonna muricata, and Clitorea ternatea. Results showed the pod husk extract of Theobroma cacao of both variants (yellow and purple) showed good antioxidant activity and antidiabetic activity with IC50 of 41.3 and 44.5 µg/mL for DPPH assay, and 41.6 and 27.7 µg/mL for α-glucosidase inhibitory assay, respectively. The pod husk also possessed most active extracts against Staphylococcus aureus with MIC of 0.62 mg/mL.  Phenolic molecules were also detected in the extracts. (50)
• Anti-Helicobacter pylori / Dried Seeds: Study evaluated the in-vitro anti-Helicobacter pylori properties of n-hexane and methanol extracts of dried seeds of Theobroma cacao in 41 H. pylori isolated from a cross-section of residents and standard H. pylori ATCC 43504 strains. The methanol extract showed varying degrees of inhibition, with ZOI between 12 mm to 17 mm on 31 of 41 clinical isolates with MICs of 80 to 90 mg/mL. Results suggest regular consumption of T. cacao seed or its products could greatly reduce the incidence of peptic ulcer and chronic gastrointestinal inflammation caused by the common gastric pathogen, H. pylori. (51)
• Inhibitory of SARS-CoV-2 Protease: Cocoa beans contain antioxidant molecules with potential to inhibit type 2 coronavirus (SARS-CoV-2), which causes a severe acute respiratory syndrome (COVID-19). In silico tests, molecular docking and quantum mechanics calculations evaluated five compounds in the series: amentoflavone, isorhoifolin, nicotiflorin, naringin, and rutin and their ability to bind to the main viral protease. Isorhoifolin and rutin stood out with more negative binding than the reference inhibitor. Results are consistent with high affinities of these molecules for the major SARS-Cov-2.  The study can be a solid starting point for future in vitro and in vivo experiments to validate the molecules and/or test similar substances as inhibitors of SARS-CoV-2 protease. (52)
• Anthelmintic / Antioxidant / Leaves: Study evaluated the antioxidant and anthelmintic activities of aqueous and ethanolic extracts of leaves. Phytochemical screening yielded tannins, saponins, glycosides, and phenols in all extracts, while flavonoids were present in the ethanolic extract only. The aqueous extract showed higher DPPH scavenging activity while the ethanolic extract showed higher ferric reducing power, total phenolic content, and anthelmintic potential against Pheretima posthuma. All extracts exhibited significant anthelmintic activity comparable to  albendazole. (53)
• Fermentation of Beans: Fermentation of cocoa beans is crucial in the development of precursors for chocolate flavor. In well fermented beans, the color of the bean change from purple to brown. The polyphenols undergo various reactions: epicatechin diffuses from storage cells, undergoes oxidation and polymerization reactions to form complex tannins.  Fermentation of cocoa beans decrease epicatechin content concentration to 2-17 mg/g. (54)
• Volatile Compounds in Seeds: Study evaluated the volatile composition of cocoa from 16 accessions from three different morphogenetic groups, namely, Criollo, Forastero, and Trinitario. Fifty-three compounds were profiled.  (see constituents above) (55)
• Antidiabetic / Antihypertensive / Antioxidant / Bean: Study evaluated the antidiabetic and antihypertensive mechanisms of Theobroma cacao through inhibition of α-amylase, α-glucosidase, angiotensin-1 converting enzyme, and oxidative stress. The extract inhibited α-amylase, α-glucosidase, and ACE activities in a dose-dependent manner and also showed dose-dependent radicals scavenging activity by DPPH assay., which could be part of the possible mechanisms for the management and/or prevention of diabetes and hypertension. (56)
• Anticancer / Antioxidant / Seed: Study evaluated the antioxidant and potential anticancer activities of seed extract. Phytochemical screening yielded cardiac glycoside, phenol, tannin, steroid, terpenoid, alkaloid, saponin, and flavonoids.  In vitro free radical scavenging activity was evaluated via DPPH, NO, lipid peroxidation and reducing power assays. The A. cepa assay revealed significant influence of the extract on mitotic cell division in a concentration dependent manner. The antiproliferative effect suggests a potential anticancer property. (57)
• Clovamide /   Antioxidant / Seed: Study of a phenolic fraction of seeds yielded clovamide (trans-clovamide,92S)-3-(3,4-dihydroxyphenyl)-2-[[(E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl]amino]propanoic acid), a naturally occurring caffeoyl conjugate and potent antioxidant.  The catechol moieties in clovamide play a major role in radical scavenging mechanism.  It contributes to antioxidant activity of cocoa, as a minor component.  It is reduced by roasting but recovered in subsequent manufacturing steps. (58)
• Antimalarial / Acute Toxicity Study / Leaves: Study evaluated the antimalarial property of Theobroma cacao leaf extract in Plasmodium berghei-infected mice using doses of 100, 200, and 400 mg/kg daily for four days. Percentage parasitaemia suppression was significant for all doses. Maximum dose of 400 mg/kbw showed highest % of parasitaemia suppression of 79.19%. Acute toxicity study  in Swiss albino rats using OECD guidelines showed no gross physical and behavioral changes and no mortality at dose level of 2000 mg/kbw. Results reinforces the justification of the plant use for treating malaria in Ghana. (61)
• Anti-Obesity / Antidiabetic / Polyphenols / Seeds: Study of raw and roasted cocoa beans of Forastero variety phenolic extracts sought to evaluate for anti-obesity, cytoprotective or insulin signaling regulator properties. Obtained preparations were in vitro investigated for PTP1B inhibition and cytoprotective activity against oxidative stress using human hepatoma HepG2 and mouse pancreatic ß-TC3 cell lines. The influence of preparations on fat tissue and antioxidant properties in vivo on rat model was studied. Results suggest the cocoa phytochemicals are potential modulators of insulin signaling, and protect beta and hepatic cells against cellular damage induced by excessive oxidative stress. Study suggests potential anti-obesity properties of roasted cocoa bean extract rich in MRP, making it a promising candidate for prevention of diabetes and associated metabolic disorders. (62)
• Anti-Inflammatory / Stem Bark: Study evaluated the anti-inflammatory activity of T. cacao stem bark ethanol extract and fractions using erythrocytes membrane stabilizing assay and carrageenan induced paw edema. The ethanol extract and its ethyl acetate fraction demonstrated anti-inflammatory activity partly by reducing neutrophil migration and inflammatory mediator production. (63)
• Tyrosinase Inhibitory Activity / Antioxidant / Pod Husk: Study sought to formulate phytosome containing cocoa pod extract, develop phytosome complex into face serum preparation, and determine antioxidant and tyrosinase inhibitory activities of the extract and formulated serum. The coco pod extract showed very strong antioxidant activity with IC50 of 17.21 ppm. Extract also showed tyrosinase inhibitory activity with IC50 of 199.98 ppm. The formulated face serum has good physical characteristic and antioxidant and tyrosinase activities that equal marketed product (Hadalabo ultimate whitening milk, Rohto, Indonesia). (64)
• Effect on Cancer Cell Survival / Antioxidative: Study sought to elucidate the possible role of cocoa in cancer therapy. Analysis revealed 34 bioactives in cocoa targeting 50 proteins regulating 21 pathways involved in cancer and oxidative stress in humans. EGFR (epidermal growth factor receptor) complex scored the highest edge count among 50 targets modulating 21 key pathways. A stable complex formation of EGFR-Hirsutrin was observed during 100 NS MD simulation. In vitro studies corroborated antioxidant activity for cocoa extract and showed significantly higher cytotoxic effect on cancer cells compared to normal cells. Results predicts anti-cancer activity for cocoa affected by hirsutrin inhibiting EGFR. (65)
• Hypoglycemic / Cocoa Autolysates: Cocoa autolysates have shown hypoglycemic activity. Cocoa autolysates produced at pH 3/5 showed most active a-amylase inhibitors. Analysis of amino acid composition showed cocoa autolysates were abundant in hydrophobic amino acids. Study suggests that besides other compounds of cocoa, its peptides and amino acids could contribute to health benefits. (66)
• Anti-Alopecia / Waste Cocoa Peels: Hair loss (alopecia) is the most common hair problem. Study evaluated the growth stimulant activity of cacao fruit peels. Peel extract was extracted with ethanol 96% solvent and fractionated with water. Various dilutions were tested in rabbits. Results showed ethanol extract of fruit peel effluent had hair growth stimulating activity starting at concentration of 15%, The n-hexane fraction showed best activity compared to other fractions and positive control (minoxidil 2%) . Waste cacao peels ethanol extract showed hair growth stimulant activity at concentrations above 15%. (67)
• Toxicological Studies on Proprietary Blend of Punica granatum and Theobroma cacao: Study evaluated LN18178 (Tesnor®), a standardized proprietary composition of aqueous ethanol extracts of Punica granatum fruit rind and Theobroma cacao seeds. In vivo and in vitro toxicological evaluation following OECD guidelines did not show any clinical signs of toxicity and morbidity in acute oral and dermal toxicity tests with medial lethal dose (LD50) of at least 5000 mg/kg and 2000 mg/kbw, respectively. Ina 90-day subchronic repeated oral dose toxicity study, there was no sign of toxicity, changes in organ weights, or changes in hematological and chemical parameters. It was neither mutagenic nor clastogenic. It did not elicit genetic toxicity in standard preclinical models. (68)
• Anti-Sickling / Antioxidant: Sickle cell anemia is a genetic disease associated with high mortality in Africa.  Study evaluated the antioxidant and anti-sickling properties of cocoa extracts from two localities of Cameroon: EFCM and EFCB. Both extracts showed high levels of polyphenols, flavonols respectively, an increase in reducing antioxidant power (FRAP), strong radical scavenging properties. The EFCM extract showed better ability to reduce RBC sickling and to protect the erythrocyte membrane from hemolysis.  Results suggest cocoa beans, especially EFCM, could be used in the management of sickle cell anemia. (70)

Drug interactions
Moderate Interactions: (1) Adenosine: The caffeine in cocoa might block the effects of adenosine (Adenocard), a drug often used in cardiac stress testing. Advise is to abstain from cocoa or other caffeine containing products at least 24 hours before a cardiac stress test. (2) Clozapine: Caffeine in cocoa may decrease the rate of break down of clozapine. (3) Dipyridamole (Persantine): Dipyridamole is used in cardiac stress testing. Stop the drug 24 hours before the stress test. (4) Ergotamine: Caffeine can increase the absorption of ergotamine. (5) Estrogen: Estrogen can decrease the breakdown of caffeine. (6) Lithium: Cocoa caffeine may increase the rate of lithium elimination. (7) MAO Inhibitors: Consumption of cocoa with MAO inhibitors (medications used for depression) might cause increased stimulation, increased heart rate, high blood pressure, nervousness, etc. (7) Hypoglycemics: By increasing blood sugar, cocoa might decrease the effectiveness of some antidiabetic medications. (8) Theophylline: Cocoa can decrease the rate of elimination of theophylline and augment its effects and increase its side effects.
Minor Interactions: Minor interactions may occur with antibiotics, birth control pills, cimetidine, disulfiram (Antabuse), fluconazole, mesiletine, verapamil. (

- Wildcrafted.
- Extracts and oils in the cybermarket.

© Godofredo U. Stuart Jr., M.D.

Updated August 2022 / March 2018 / November 2016

Photos / Content ©Godofredo Stuart / StuartXchange
OTHER IMAGE SOURCE: B&W Drawing / Fig. 261. - Theobroma Cacao / Chocolate Tree / Chest of Books
IMAGE SOURCE: File:Theobroma cacao Blanco2.275-cropped.jpg / Francisco Manuel Blanco (O.S.A.) / Flora de Filipinas / 1880-1883 Public Domain / Modifications by Carol Spears / Wikimedia Commons

Additional Sources and Suggested Readings
Medicinal value of chocolate explored by scientists / Mongabay
Hypoglycaemic Properties of Malaysian Cocoa (Theobroma Cacao) Polyphenols-Rich Extract / Ruzaidi, A., Abbe Maleyki et al / International Food Research Journal 15(3), (2008)
Effects of polyphenol substances derived from Theobroma cacao on gastric mucosal lesion induced by ethanol / OSAKABE N., SANBONGI C et al / Bioscience, biotechnology, and biochemistry / 1998, vol. 62, no8, pp. 1535-153
Pentameric Procyanidins Isolated from Theobroma cacao Seeds Selectively Downregulate ErbB2 in Human Aortic Endothelial Cells / Thomas P. Kenny et al / Experimental Biology and Medicine, 2004; 229: pp 255-263 / DOI: 10.1177/153537020422900306

Effect of Theobroma cacao flavonoids on immune activation of a lymphoid cell line / Emma Ramiro, Ángels Franch, Cristina Castellote, Cristina Andre ́s-Lacueva, Maria Izquierdo-Pulido and Margarida Castell* / British Journal of Nutrition (2005), 93, 859–866 / DOI: 10.1079/BJN20051443
Dietary flavanols and procyanidin oligomers from cocoa (Theobroma cacao) inhibit platelet function / Karen J Murphy, Andriana K Chronopoulos et al / American Journal of Clinical Nutrition, Vol. 77, No. 6, 1466-1473, June 2003
Antioxidative Polyphenols Isolated from Theobroma cacao / Chiaki Sanbongi et al / J. Agric. Food Chem., 1998, 46 (2), pp 454–457 / DOI: 10.1021/jf970575o
Flavonoids from Theobroma cacao Down-Regulate Inflammatory Mediators / Emma Ramiro et al / J. Agric. Food Chem., 2005, 53 (22), pp 8506–8511 / DOI: 10.1021/jf0511042
Protective Activity of Theobroma cacao L. Phenolic Extract on AML12 and MLP29 Liver Cells by Preventing Apoptosis and Inducing Autophagy / Marco Arlorio et al / J. Agric. Food Chem., 2009, 57 (22), pp 10612–10618 / DOI: 10.1021/jf902419t
Sorting Theobroma names / Porcher Michel H. et al. 1995 - 2020 / MULTILINGUAL MULTISCRIPT PLANT NAME DATABASE
Polyphenols in cocoa (Theobroma cacao L.) / C.L. Hii, C.L. Law, S. Suzannah, Misnawi and M. Cloke / As. J. Food Ag-Ind. 2009, 2(04), 702-722
Antioxidant and biological activity of phenolic pigments from Theobroma cacao hulls extracted with supercritical CO2 / M. Arlorio, J.D. Co ̈ısson, F. Travaglia et al / Food Research International 38 (2005) 1009–1014
Theobroma cacao L., the Food of the Gods: A scientific approach beyond myths and claims / M. Rusconi∗, A. Conti / Pharmacological Research 61 (2010) 5–13
The Protective Effect of Cocoa (Theobroma cacao L.) in Colon Cancer / Yazan Yazan Ranneh*, Faisal Ali and Norhaizan Mohd Esa / J Nutr Food Sci 3:193. doi: 10.4172/2155-9600.1000193
Production of Activated Carbon from Cocoa (Theobroma cacao) Pod Husk / Gerardo Cruz*, Minna Pirilä, Mika Huuhtanen, Lili Carrión, Emilio Alvarenga and Riitta L Keiski / J Civil Environment Engg 2:109. / doi:10.4172/2165-784X.1000109
Farm and Forestry Production and Marketing Profile for Cacao (Theobroma cacao)
/ Prakash Hebbar, H.C. Bittenbender, and Daniel O'Doherty / Agroforestry
Contemporary Reviews in Cardiovascular Medicine: Cocoa and Cardiovascular Health / Roberto Corti, MD*; Andreas J. Flammer, MD*; Norman K. Hollenberg, MD, PhD; Thomas F. Lüscher, MD / Circulation, 2009; 119: 1433-1441 / doi: 10.1161/​CIRCULATIONAHA.108.827022
Cacao: Theobroma cacao (LINN.) / A Modern Herbal by Mrs. M Grieve / Botanical.com
(19) /
Chocolate Lover? Compound Inside Cocoa Beans Causes Parkinson's - GMO Cocoa Trees To Increase Its Concentration / NATASHA LONGO / April 12, 2013 / WHN Forum for Anti-Aging & Regenerative Disease
Effect of Theobroma cacao flavonoids on immune activation of a lymphoid cell line / Emma Ramiro, Angels Franch, Cristina Castellote, Cristina Andre ́s-Lacueva, Maria Izquierdo-Pulido and Margarida Castell* / British Journal of Nutrition (2005), 93, 859–866 / DOI: 10.1079/BJN20051443
Anticancer Agents from Non-Edible Parts of Theobroma cacao / Zainal B, Abdah MA*, Taufiq-Yap YH, Roslida AH and Rosmin K / Natural Products Chemistry & Research, 2:134. / doi: 10.4172/2329-6836.1000134
Effect of roasting conditions on the fat, tocopherol, and phytosterol content and antioxidant capacity of the lipid fraction from cocoa beans of different Theobroma cacao L. cultivars / Joanna Oracz*, Ewa Nebesny andDorota Żyżelewicz / European Journal of Lipid Science and Technology, Volume 116, Issue 8, pp 1002–1014, August 2014 / DOI: 10.1002/ejlt.201300474
Roasting impact on the contents of clovamide (N-caffeoyl-L-DOPA) and the antioxidant activity of cocoabeans (Theobroma cacao L.) / Marco Arlorio, Monica Locatelli *, Fabiano Travaglia, Jean-Daniel Co ̈ısson, Erika Del Grosso, Alberto Minassi, Giovanni Appendino, Aldo Martelli / Food Chemistry 106 (2008) 967–975
Theobroma cacao / Synonyms / The Plant List
Cocoa Bioactive Compounds: Significance and Potential for the Maintenance of Skin Health / Giovanni Scapagnini,* Sergio Davinelli, Laura Di Renzo, Antonino De Lorenzo, Hector Hugo Olarte, Giuseppe Micali, Arrigo F. Cicero, and Salvador Gonzalez / Nutrients. 2014 Aug; 6(8): 3202–3213 / doi: 10.3390/nu6083202
Effects of the Intake of Natural Cocoa Powder on Some Biochemical and Haematological Indices in the Rat
/ F K Abrokwah, K A Asamoah, and P K A Esubonteng / Ghana Med J. 2009 Dec; 43(4): 164–168
In vitro Antioxidant Assay of Cocoa (Theobroma cacao) Oil and Cake / Ganiyat Kehinde Oloyede and Sunday Felix Abimbade / AU J.T. 17(3) 101-108 (Jan. 2014)
Cocoa and Chocolate: Composition, Bioavailability, and Health Implications / Andrea T. Borchers, Carl L. Keen, Sandra M. Hannum, and M. Eric Gershwin / Journal of Medicinal Food. June 2000, 3(2): 77-105. / doi:10.1089/109662000416285.
ESTIMATION OF PROTEIN CONTENT AND PHYTOCHEMICALS STUDIES IN COCOA FRUIT OUTER COVERING / Dr. D. Sailaja, P. Srilakshmi*, K. Puneeth and C. Ramya Krishna / International Journal of Plant, Animal, and Environmental Sciences, Volume-5, Issue-1, Jan-Mar-2015
Herb-Drug Interactions: Cocoa / Medicinal plants and their uses (medicinal herbs): Chemical Components, Main Actions, Clinical Use, Dosage Range, Toxicity, Adverse Reactions, Significant Interactions, Contraindications and Precautions, Pregnancy Use, Practice Points, Patient Counselling / Medicinal Plants 2015
Antimicrobial activity of fermented Theobroma cacao pod husk extract / R.X. Santos, D.A. Oliveira, G.A. Sodré, G. Gosmann, M. Brendel and C. Pungartnik / Genetics and Molecular Research 13 (3): 7725-7735 (2014)
The Chemical Constituents and Biological Activities of Stem Bark Extract of Theobroma Cacao / Nwokonkwo D. C. & Okeke, G. N. / Global Journal of Science Frontier Research: E Interdiciplinary, Volume 14, Issue 4, 2014
Cocoa consumption alters the global DNA methylation of peripheral leukocytes in humans with cardiovascular disease risk factors: A randomized controlled trial / Crescenti A, Solà R, Valls RM, et al. / PLoS One. 2013;8(6):e65744. / doi: 10.1371/journal.pone.0065744.
The Potential of Cocoa Extract as a Hypoglycemic Agent / Chee Beng Jin, Muhajir Hamid, Amin Ismail and Chong Pei Pei / J. Trop. Med. Plants, Vol 13, No 2, Dec 2012
Hypoglycaemic Properties of Malaysian Cocoa (Theobroma Cacao) Polyphenols Extract / A. RUZAIDI , I. AMIN, A.G. NAWALYAH, H. MUHAJIR, M.B.S. PAULIENA and M.S. MUSKINAH
Immunomodulatory properties of cacao extracts – potential consequences for medical applications / Kathrin Becker, Simon Geisler, Florian Ueberall, Dietmar Fuchs* and Johanna M. Gostner

Short-Term Capacities of Ethanolic Theobroma Cacao Bean Extract to Ameliorate Oxidative Stress, Hyperglycemia and Dyslipidemia in Alloxan-Induced Diabetic Rats / Paul Chidoka Chikezie / J Invest Biochem. 2015; 4(1): 23-29doi: 10.5455/jib.20150504014936
Ethnobotanical Survey of Medicinal Plants Used In the Treatment of Skin Diseases in Abeokuta South Local Government of Ogun State Nigeria / Adeogun, I.I., O.O. Fawibe, A.A. Ajiboye, D.A. Agboola* / Asian Journal of Pharmaceutical Technology and Innovation
Cocoa Interactions / WebMD
Theobroma cacao: Review of the Extraction, Isolation, and Bioassay of Its Potential Anti-cancer Compounds / Zainal Baharum, Abdah Md Akim, Taufig Yap Yun Hin, Roslida Abdul Hamid, and Rosmin Kasran / Trop Life Sci Res., Feb 2016; 27(1): pp 21-42

Cacao: Theobroma spp / Sacred Earth
Theobroma cacao / Useful Tropical Plants
Studies on the Effect of Polyphenol of Cocoa (Theobroma cacao L.) Seeds on Specific Carbohydrate-Degrading Enzymes in Streptozotocin-Induced Diabetic Rats / Christianah Dare et al / Greener Journal of Biochemistry and Biotechnology / DOI: 10.15580/GJBB.2014.1.040414176
The Antimalarial Potential of Three Ghanaian Medicinal Plants / Gustav Komlaga, Sandrine Cojean, Mehdi Beniddir A, Rita Dicksona, Pierre Champy, Merlin Lincoln Kwao Mensah, soulaf Suyyagh-Albouz, Jonathan Jato, and Philippe Loiseau M / DOI: 10.21767/2472-0151.10004
Antimicrobial activity and cytotoxicity of Theobroma cacao extracts / Nidhi Singh, Shreyan Datta, Abhirup Dey, Akash Roy Chowdhury and Jayanthi Abraham / Der Pharmacia Lettre, 2015, 7(7): pp 287-294
Antitumor activity against murine lymphoma L5178Y model of proteins from cacao (Theobroma cacao L.) seeds in relation with in vitroantioxidant activity / Ana M PrezaMaría E JaramilloAna M PueblaJuan  C MateosRodolfo Hernández and Eugenia Lugo / BMC Complementary and Alternative MedicineThe official journal of the International Society for Complementary Medicine Research (ISCMR) 2010, 10:61 / https://doi.org/10.1186/1472-6882-10-61
A comparative phytochemical analysis of cocoa and green tea / R. Subhashini, U.S. Mahadeva Rao, P.Sumathi and Gayathri Gunalan* / Indian Journal of Science and Technology Vol. 3 No. 2 (Feb 2010)
Dark Chocolate: 70-85% Cacao Solids / USDA: National Nutrient Data Base
In Vitro Antioxidant and Antiproliferative Activities of Methanolic Plant Part Extracts of Theobroma cacao
/ Zainal Baharum, Abdah Md Akim, Rosmin Kasran et al / Molecules, 2014; 109(11) : pp 18317-18331 /
DOI: 10.3390/molecules191118317
In vitro studies of antioxidant, antidiabetic, and antibacterial activities of Theobroma cacao, Anonna muricata, and Clitorea ternatea / Anastasia Wheni Indrianingsih, Takeshi Katayama et al / Biocatalysis and Agricultural Biotechnology, 2021; Vol 33: 101995 / DOI: 10.1016/j.bcab.2021.101995
Susceptibility testing and bactericidal activities of Theobroma cacao Linn. (Cocoa) on Helicobacter pylori in an in vitro study / Temitope O Lawal, Bolanle A Adeniyi et al / Journal of Herbal Medicine, Dec 2014; 4(4): pp 201-207 / DOI: 10.1016/j.hermed.2014.09.004
Theobroma cacao L. compounds: Theoretical study and molecular modeling as inhibitors of main SARS-CoV-2 protease / Osvaldo Yañez, William Tiznado et al / Biomedicine & Pharmacotherapy, Aug 2021; Vol 140: 111764 / DOI: 10.1016/j.biopha.2021.111764
In vitro anthelmintic and antioxidant activities of the leaf extracts of Theobroma cacao L. / Olukemi Osukoya, Adewale Fadaka, Olusola Adewale et al / AIMS Agriculture and Food, 2019; 4(3): pp 568-577 / DOI: 10.3934/agrfood.2019.3.568
Theobroma cacao L., the Food of the Gods: A scientific approach beyond myths and claims / N Rusconi, A Conti et al / Pharmacological Research,, Jan 2010; 61(1): pp 5-13 / DOI: 10.1016/j.phrs.2009.08.008
Characterization of volatile compounds in Criollo, Forastero, and Trinitario cocoa seeds (Theobroma cacao L.) in China / Xiao-Wei Qin, Jian-Xiong Lai et al / International Journal of Food Properties, 2017, 20(10): pp 2261-2275 / DOI: 10.1080/10942912.2016.1236270
In Vitro Studies on the Antioxidant Property and Inhibition of α-Amylase, α-Glucosidase, and Angiotension 1-Converting Enzyme by Polyphenol-Rich Extracts from Cocoa  (Theobroma cacao) Bean
/  Ganiyu Oboh, Ayokunle O Ademosun, Kuburat O Longe et al / Pathology Research International, Vol 2014,  Article ID 549287 / DOI: 10.1155/2014/549287
Hydro-ethanol seed extract of Theobroma cacao exhibits antioxidant activities and potential anticancer property / Osaretin A T Ebuehi, Chinonye Anams et al / Journal of Food Biochemistry, 43(4): e12767 /
DOI: 10.1111/jfbc.12767
Antioxidant studies by hydrodynamic voltammetry and DFT, quantitative analyses by FPLC-DAD of clovamide, a natural phenolic compound found in Theobroma cacao L. beans / Naike Ye, Miriam Rossi et al / Food Chemistry, March 2021; Vol 341, Part 2: 128260 / DOI: 10.1016/j.foodchem.2020.128260
Medicinal plants used by traditional practitioners in two selected villages of Ramon Magsaysay, Zamboanga del Sur / Lady Jane G Morilla, Cesar G Demayo / Pharmacophore, 2019; 10(1): pp 84-92 / ISSN: 2229-5402
Nutritional Composition of Ten Ethnobotanicals Used for the Treatment of Anaemia in Southwest Nigeria
/ I T Gbadamosi, J O Moody, A O Yekini / European Journal of Medicinal Plants, 2012; 2(2): pp 140-150
Antimalarial Property and Acute Toxicity of the Leaves of Theobroma cacao L.
/ Gustav Komlaga, Arnold Donkor Forkuo, Nadiatu Suleman et al / Evidence-Based Complementary and Alternative Medicine, Volume 2021, Article ID 2852442 / DOI: 10.1155/2021/2852442
Cocoa bean (Theobroma cacao L.) phenolic extracts as PTP1B inhibitors, hepatic HepG2 and pancreatic ß-TC3 cell cytoprotective agents and their influence on oxidative stress in rats
/ Dorota Zyzelewicz, Adam Jurgonski et al / Food Research International, Nov 2016; Vol 82, Part 2: pp 946-957 /
DOI: 10.1016/j.foodres.2016.01.009
Anti-inflammatory activity of Theobroma cacao L. stem bark ethanol extract and its fractions in experimental models / Sabitiu A Oyeleke, Olusegun George Ademowo et al /   Journal of Ethnopharmacology, August 2018; Vol 222: pp 239-248 /  DOI: 10.1016/j.jep.2018.04.050
Antioxidant and tyrosinase inhibitory activity of face serum containing cocoa pod husk phytosome (Theobroma cocoa L.) / Sani Ega Priani, Saskara Aprilia et al / Journal of Applied Pharmaceutical Science, 9(10):  pp 110-115 / DOI: 10.7324/JAPS.2019.91015 / ISSN: 2231-3354
Network pharmacology and in vitro testing of Theobroma cacao extract's antioxidative activity and its effects on cancer cell survival / Priyanka P Patil, Vishal S Patil, Subarna Roy et al / PLOS ONE /
DOI: 10.1371/journal.pone.0259757
Hypoglycemic effects of cocoa (Theobroma cacao L.) autolysates / Bahareh Sarmadi, Amin Ismail et al / Food Chemistry, Sept 2012; 134(2): pp 905-911 / DOI: 10.1016/j.foodchem.2012.02.202
Anti-alopecia activity of waste cacao (Theobroma cacao L.) peels /  Mustarichie Resmi, Aliya Nur Hasanah /  Drug Invention Today, 2019; 11(9): pp 2194-2199
Traditional Knowledge on Medicinal Plants Use by Ethnic Communities in Douala, Cameroon / Mpondo Mpondo Emmanuel, Dibong Siegfried Didier / European Joiurnal of Medicinal Plants, 2012; 2(2): pp 159-176
Toxicological Assessments of a Proprietary Blend of Punica granatum Fruit Rind and Theobroma cacao Seed Extracts: Acute, Subchronic, and Genetic Toxicity Studies /  Ravi Kumar Madireddy, Krishanu Sengupta et al / Journal of Toxicology, Volume 2022; Article ID 3903943 /  DOI: 10.1155/2022/3903943
In vitro evaluation of antioxidant and anti-sickling properties   pf Theobroma cacao (Sterculiaceae) extracts from East and South regions in Cameroon / Natacha L Yembeau,, Proisper C N Biapa et al / Investigational Medicinal Chemistry & Pharmacology, 2018; 1(1)

DOI: It is not uncommon for links on studies/sources to change. Copying and pasting the information on the search window or using the DOI (if available) will often redirect to the new link page. (Citing and Using a (DOI) Digital Object Identifier)

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